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BacterStain™ Lugol
- A reagent is recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms
- Code: 04005
- Packaging: 100ml/bottle or according to the customer’s request..
- Storage Conditions: 18 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
INTENDED USE
- BacterStrain™ Lugol is an iodine solution used in the Gram staining method to form a complex with Crystal Violet, helping to retain the purple color in Gram-positive bacteria. It is also used in microbiological research, to test the precipitation of substances, and as an antiseptic in medical applications.
PRINCIPLES
- BacterStrain™ Lugol contains iodine and potassium iodide, which, when in contact with Crystal Violet, form a complex with the dye, helping it bind tightly to the cell wall of Gram-positive bacteria. This complex is resistant to decolorization by acid alcohol, allowing Gram-positive bacteria to retain the purple color while Gram-negative bacteria do not.
Used in Gram Strain Kit
- Prepare a thin smear on clear, dry glass slide.
- Allow it to air dry and fix by gentle heat
- Flood with BacterStain™ Crystal Violet (Code: 04007) for 1 minute.
- Wash with tap water
- Flood the smear with BacterStain™ Lugol (Code: 04005). Allow it to remain for 1 minute
- Decolourize with BacterStain™ Alcohol (Code: 04011) until the blue dye no longer flows from the smear
- Wash with tap water
- Counter stain with BacterStain™ Safranine (Code: 04008) for 1 minutes and rinses off with water
- Allow the slide to air dry or blot dry between sheets of clean bibulous paper and examine under oil immersion objective
RESULTS
- Gram-positive bacteria stain dark violet with gentian: Staphylococcus, Streptococcus, Pneumococcus, Diphtheria bacillus, Bacillus anthracis.
- Gram-negative bacteria do not stain violet with gentian, so they appear pink-red with fuchsin: Neisseria gonorrhoeae, Neisseria meningitidis, Escherichia coli, Shigella, Salmonella, Vibrio cholerae.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Conditions | Expected results |
| Staphylococcus aureus ATCC 25923 | oil immersion lens (100x) | dark violet with gentian |
| E. coli ATCC 35218 | pink-red with fuchsin |
For 1 liter of strain
| Iodine | 3 g |
| KI | 6 g |
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterStrain™ Lugol | 04005 | 100ml/bottle or according to the customer’s request.. |
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