Vn
BacterStain™ Crystal Violet
- The dye used to stain tissues and used in the Gram method to classify bacteria
- Code: 04007
- Packaging: 100ml/bottle or according to the customer’s request..
- Storage Conditions: 18 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
INTENDED USE
- BacterStrain™ Crystal Violet is primarily used in the Gram staining method to differentiate between Gram-positive and Gram-negative bacteria. It is also used in cell staining, histological research, and enzyme activity determination.
PRINCIPLES
- BacterStrain™ Crystal Violet is a cationic dye that binds to the bacterial cell wall, particularly to the polysaccharides and peptidoglycan in Gram-positive bacteria. During staining, Gram-positive bacteria retain the purple color due to their strong cell wall structure, while Gram-negative bacteria lose the color and are subsequently counterstained with safranin.
Used in Gram Strain Kit
- Prepare a thin smear on clear, dry glass slide.
- Allow it to air dry and fix by gentle heat
- Flood with BacterStain™ Crystal Violet (Code: 04007) for 1 minute.
- Wash with tap water
- Flood the smear with BacterStain™ Lugol (Code: 04005). Allow it to remain for 1 minute
- Decolourize with BacterStain™ Alcohol (Code: 04011) until the blue dye no longer flows from the smear
- Wash with tap water
- Counter stain with BacterStain™ Safranine (Code: 04008) for 1 minutes and rinses off with water
- Allow the slide to air dry or blot dry between sheets of clean bibulous paper and examine under oil immersion objective
RESULTS
- Gram-positive bacteria stain dark violet with gentian: Staphylococcus, Streptococcus, Pneumococcus, Diphtheria bacillus, Bacillus anthracis.
- Gram-negative bacteria do not stain violet with gentian, so they appear pink-red with fuchsin: Neisseria gonorrhoeae, Neisseria meningitidis, Escherichia coli, Shigella, Salmonella, Vibrio cholerae.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Conditions | Expected results |
| S. aureus ATCC 25923 | oil immersion lens (100x) | dark violet with gentian |
| E. coli ATCC 35218 | pink-red with fuchsin |
For 1 liter of strain
| Crystal Violet | 20 g |
| KI | 6 g |
| Ethanol | 200 mL |
| Amonium oxlate | 8 g |
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterStrain™ Crystal Violet | 04007 | 100ml/bottle or according to the customer’s request.. |
Related products
BacterStain™ Auramine Solution
- A fluorescent dye used in microbiology for the detection of acid-fast bacteria (AFB) through fluorescence microscopy
- Code: 04012
- Packaging: 100ml/bottle or according to the customer’s request..
- Storage Conditions: 18 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
BacterStain™ Gram Stain Kit
- A set of reagents used in a technique for the differentiation of Gram-negative and Gram-positive microorganisms.
- Code: 04001
- Packaging: Crystal Violet; Lugol; Safranin; Alcohol: 4 bottles/box 100ml or as per customer request.
- Storage Conditions: 18–25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
BacterStain™ Ziehl Neelsen Stain Kit
- A set of reagents used for the dectection of acid-alcohol resistant bacteria.
- Code: 04002
- Packaging: Alcohol acid; Carbol fuchsin; Methylene blue: 3 bottles/box 100ml or as per customer request.
- Storage Conditions: 18–25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
BacterStain™ Giemsa
- A dye used for microbiology and hematology, commonly employed for staining cell and tissue samples in bacteriological, virological, and hematological studies.
- Code: 04003
- Packaging: 100ml/bottle or according to the customer’s request..
- Storage Conditions: 18–25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
BacterStain™ Safranine
- A synthetic red dye used in microbiology and histology.
- Code: 04008
- Packaging: 100ml/bottle or according to the customer’s request..
- Storage Conditions: 18 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
BacterStain™ Eosin
- A synthetic dye used in various staining techniques, particularly in histology and cytology.
- Code: 04010
- Packaging: 100ml/bottle or according to the customer’s request..
- Storage Conditions: 18 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
BacterStain™ Alcohol
- An solvent used in staining methods, such as Gram staining, to decolorize bacteria, fix, and dry cell samples
- Code: 04011
- Packaging: 100ml/bottle or according to the customer’s request..
- Storage Conditions: 18 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
BacterStain™ Lugol
- A reagent is recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms
- Code: 04005
- Packaging: 100ml/bottle or according to the customer’s request..
- Storage Conditions: 18 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
BacterStain™ Alkaline Methylene Blue
- A reagent is recommended for use in clinical diagnosis of human clinical specimens and for bacteriological and histological research purposes using human-derived samples.
- Code: 04006
- Packaging: 100ml/bottle or according to the customer’s request..
- Storage Conditions: 18 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
BacterStain™ Carbon Fuchsin
- The dye is used in the acid-fast staining method.
- Code: 04004
- Packaging: 100ml/bottle or according to the customer’s request..
- Storage Conditions: 18 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.