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BacterPlate™ Salmonella Shigella Agar (SS)
- Ready-to-use medium on 90mm plates for the selective culture of Salmonella and Shigella. Applied for culturing stool samples.
- Code: 05019
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Salmonella Shigella Agar (SS) is used for the isolation of Salmonella and Shigella in fecal material. It can also be used as a second media of isolation in the context of standardized methods for the detection of Salmonella.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLE
- BacterPlate™ Salmonella Shigella Agar (SS) is a moderately selective medium in which Gram-positive bacteria are inhibited by bile salts, brilliant green and sodium citrate. The high concentrations of citrate and sodium thiosulfate limit the development of coliform bacteria and prevent the invasion of the medium by Proteus. Fermentation of lactose to acid is revealed by the formation of red colonies in the presence of neutral red. Lactosenegative bacteria form colorless colonies. In the presence of thiosulfate and ferric citrate, hydrogen sulfide producers form colonies with a black center.
- Dry the plates in an incubator, covers partially removed.
- Inoculate by streaking the enrichment media used on the surface of the plates
- Incubate at 37 °C for 24 to 48 hours
RESULTS
- Salmonella species not fermenting lactose form colorless, transparent colonies, with or without a black center (H2S production).
- Shigella are colorless.
- Colonies of coliform bacteria are red or pink.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Standard Microorganism Strain | Incubation Conditions | Expected result |
| S. typhimurium ATCC 14028 | 18 – 24 hours at 35 – 37 oC | Good growth, colorless colonies with black centers |
| S. flexneri ATCC 12022 | Good growth, colorless colonies | |
| E. coli ATCC 25922 | Partial inhibition, pink colonies | |
| S. aureus ATCC 25923 | Inhibited, no growth |
For 1 liter of medium
| Pancreatic digest of meat | 5,0 g |
| Meat extract | 5,0 g |
| Lactose | 10,0 g |
| Bile salts | 8,5 g |
| Sodium citrate | 10,0 g |
| Sodium thiosulfate | 8,5 g |
| Ferric ammonium citrate | 1,0 g |
| Neutral red | 25,0 mg |
| Brilliant green | 0,33 mg |
| Agar | 15,0 g |
pH of the ready-to-use medium at 250C: 7,0 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Salmonella Shigella Agar (SS) | 05019 | 10 plates/ box or as per customer request. |
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