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INTENDED USE
- BacterStrain™ Safranine is a synthetic red dye used in the Gram staining method, primarily as a counterstain for Gram-negative bacteria.
PRINCIPLES
- After Gram-negative bacteria are decolorized by alcohol, they no longer retain the Crystal Violet color and are counterstained with Safranine. As a result, Gram-negative bacteria will appear pink or red, while Gram-positive bacteria retain their purple color, providing a clear distinction between the two types of bacteria.
Used in Gram Strain Kit
- Prepare a thin smear on clear, dry glass slide.
- Allow it to air dry and fix by gentle heat
- Flood with BacterStain™ Crystal Violet (Code: 04007) for 1 minute.
- Wash with tap water
- Flood the smear with BacterStain™ Lugol (Code: 04005). Allow it to remain for 1 minute
- Decolourize with BacterStain™ Alcohol (Code: 04011) until the blue dye no longer flows from the smear
- Wash with tap water
- Counter stain with BacterStain™ Safranine (Code: 04008) for 1 minutes and rinses off with water
- Allow the slide to air dry or blot dry between sheets of clean bibulous paper and examine under oil immersion objective
RESULTS
- Gram-positive bacteria stain dark violet with gentian: Staphylococcus, Streptococcus, Pneumococcus, Diphtheria bacillus, Bacillus anthracis.
- Gram-negative bacteria do not stain violet with gentian, so they appear pink-red with fuchsin: Neisseria gonorrhoeae, Neisseria meningitidis, Escherichia coli, Shigella, Salmonella, Vibrio cholerae.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Conditions | Expected results |
| S. aureus ATCC 25923 | oil immersion lens (100x) | dark violet with gentian |
| E. coli ATCC 35218 | pink-red with fuchsin |
For 1 liter of strain
| Safranin O | 2,5 g |
| Ethanol | 100 mL |
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterStrain™ Safranine | 04008 | 100ml/bottle or according to the customer’s request.. |
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