Vn
INTENDED USE
- BacterTest™ FeCl3 is a reagent kit combined with the PAD biochemical medium or paper plate to perform the Phenylalanie deaminase (PAD) test. The Phenylalanine test is based on the detection of phenylpyruvic acid in the medium. If phenylpyruvic acid is present in the medium (positive test), a blue color of iron (III) phenylpyruvate appears.
PRINCIPLES
- In the Phenylalanie deaminase test (PPA), bacteria capable of producing the enzyme phenylalanine deaminase will deamine phenylalanine, converting it to phenylpyruvic acid (PPA). PPA can then react with FeCl₃, producing a characteristic green complex.
- Bacteria are cultured in a prepared medium containing phenylalanine (Phenylalanine Agar) after 24 – 48 hours at 37°C.
- Adding 1 – 2 drops of FeCl₃ to the bacterial culture medium.
- Observing the color change of the medium, reading and recording the results
RESULTS
- Observe the color change of the environment:
- Green= Positive (bacteria are able to degrade phenylalanine)
- ellow or no change= Negative (bacteria are not able to degrade phenylalanine).
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Growth | Phenylalanie deaminase test |
| E. coli ATCC 35218 | Good growth | (–): Green |
| P. mirabilis ATCC 14153 | Good growth | (+): Yellow or no change |
Refer to the components.
| FeCl₃ | 12 g |
| axit clohydric (HCl) | 2,5 mL |
| distilled water | 97,5 mL |
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterStrain™ FeCl3 | 06002 | 100mL/ dropper bottle or as per customer request. |
Related products
BacterPlate™ XLD Agar
- Ready-to-use medium on 90mm plates for detecting Salmonella and Shigella.
- Code: 05043
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
BacterPlate™ R2A Agar
- Ready-to-use medium on 90mm plates for the enumeration of microorganisms in water
- Code: 05057
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterBagTM Dry Maximum Recovery Diluent
- High-quality dry medium, packaged in a transparent, lightweight, and convenient plastic bag. Protective and isotonic medium used for maximal recovery of microorganisms from a variety of sources.
- Code: 10016
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterBag™ Dry Half Fraser Broth
- Dry powder form of Half Fraser Broth medium, used for the enrichment of Listeria monocytogenes.
- Code: 10014
- Packaging: Equivalent to 20L of medium per bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 36 months from the date of manufacture.
BacterBag™ Buffered Peptone Water (BPW)
- The non-selective enrichment medium is used in the pre-enrichment step of the Salmonella testing process.
- Code: 10001
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterContact™ Pro Tryptic Soy Agar + Neutralizer A
- Ready-to-use medium on 60mm plates for surface microbiological sampling
- Code: 12016
- Packaging: Three-layer packaging irradiated with 10 discs per box, or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 9 months from the date of manufacture.
BacterContact™ Pro Tryptic Soy Agar
- Ready-to-use medium on 60mm plates for surface microbiological sampling
- Code: 12023
- Packaging: Three-layer packaging irradiated with 10 discs per box, or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 9 months from the date of manufacture.
BacterPlate™ TCBS Agar
- Ready-to-use medium on 90mm plates for isolating and selecting Vibrio. Differentiates Vibrio cholerae from other Vibrio species based on sucrose fermentation capability.
- Code: 05022
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterTube™ Lysin decarboxylase (LDC)
- The test medium used to differentiate Salmonella arizona from the Bethesda Ballerup group of Enterobacteriaceae.
- Code: 08030
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
BacterContact™ Pro Sabouraud Dextrose Agar + LT
- Ready-to-use medium on 60mm plates for surface microbiological sampling with added neutralizers to inactivate surface disinfectants
- Code: 12020
- Packaging: Three-layer packaging irradiated with 10 discs per box, or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 9 months from the date of manufacture.