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INTENDED USE
- BacterTest™ FeCl3 is a reagent kit combined with the PAD biochemical medium or paper plate to perform the Phenylalanie deaminase (PAD) test. The Phenylalanine test is based on the detection of phenylpyruvic acid in the medium. If phenylpyruvic acid is present in the medium (positive test), a blue color of iron (III) phenylpyruvate appears.
PRINCIPLES
- In the Phenylalanie deaminase test (PPA), bacteria capable of producing the enzyme phenylalanine deaminase will deamine phenylalanine, converting it to phenylpyruvic acid (PPA). PPA can then react with FeCl₃, producing a characteristic green complex.
- Bacteria are cultured in a prepared medium containing phenylalanine (Phenylalanine Agar) after 24 – 48 hours at 37°C.
- Adding 1 – 2 drops of FeCl₃ to the bacterial culture medium.
- Observing the color change of the medium, reading and recording the results
RESULTS
- Observe the color change of the environment:
- Green= Positive (bacteria are able to degrade phenylalanine)
- ellow or no change= Negative (bacteria are not able to degrade phenylalanine).
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Growth | Phenylalanie deaminase test |
| E. coli ATCC 35218 | Good growth | (–): Green |
| P. mirabilis ATCC 14153 | Good growth | (+): Yellow or no change |
Refer to the components.
| FeCl₃ | 12 g |
| axit clohydric (HCl) | 2,5 mL |
| distilled water | 97,5 mL |
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterStrain™ FeCl3 | 06002 | 100mL/ dropper bottle or as per customer request. |
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