BacterTube™ BHI with Chloramphenicol

INTENDED USE

BacterTube™ BHI with Chloramphenicol is an enriched medium designed to support the growth of both aerobic and anaerobic microorganisms. It is particularly useful for isolating pathogenic fungi from clinical samples or environments heavily contaminated with bacterial and saprophytic fungal flora.

PRINCIPLES

BacterTube™ BHI with Chloramphenicol is an enriched medium designed to support the growth of both aerobic and anaerobic microorganisms. It is particularly useful for isolating pathogenic fungi from clinical samples or environments heavily contaminated with bacterial and saprophytic fungal flora.

Heat the tip of the inoculation loop until red in the flame, then gently heat the handle, and hold the loop vertically to ensure even heating.
Inoculate the sample onto the surface of the slant agar by lightly placing the tip of the loop on the surface at the bottom of the tube.
Then, streak the sample in a zig-zag pattern from the bottom of the test tube up to the top of the slant agar.
Incubate under aerobic conditions at 35 – 37°C for 16 – 24 hours

RESULTS

After incubating for the required time, typically 18-24 hours, observe the bacterial colonies growing on the surface of the medium.
To identify the isolated bacteria, further appropriate tests must be conducted.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Candida albicans ATCC 10231	18 – 24 hours, 35 – 37°C	Good growth
Eschirichia coli ATCC 25922	18 – 24 hours, 35 – 37°C	Partial to complete inhibition

For 1 liter of medium

Pancreatic Digest of Casein	16,0 g
Brain Heart Infusion	8,0 g
Peptic Digest of Animal Tissue	5,0 g
Sodium chloride	5,0 g
Disodium Phosphate	2,5 g
Dextrose	2,0 g
Chloramphenicol	0,1 g
Agar	13,5 g