INTENDED USE
- BacterTube™ Cary Blair is recommended for collection and shipment of clinical specimens.
PRINCIPLES
- BacterTube™ Cary Blair is prepared with minimal nutrients to increase the survival of the organisms without multiplying. Sodium thioglycollate is incorporated in the medium to provide a low oxidation-reduction potential. The pH of the medium is relatively alkaline which minimizes the bacterial destruction due to the formation of acid. Phenol red is added as indicator which is red at alkaline pH, while yellow at acidic pH. Medium can maintain viability of fastidious microorganisms for only a short period of time. It is recommended that best results are obtained by direct inoculation of isolate on enriched medium at the same time specimen is inoculated into transport medium.
- Ensure the medium reaches room temperature before use.
- Collect clinical samples following standard protocols.
- Insert or place the sample into the BacterTube™ Cary Blair.
- Transport the sample to the laboratory within 24–48 hours under cool conditions
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strain | Incubation conditions | Expected result |
| Salmonella typhimurium ATCC 14028 | 18 – 24 hours at 35 – 370C | Good Growth on Subculture Medium |
| V.parahaemolyticus ATCC 17802 | Good Growth on Subculture Medium | |
| Shigella flexneri ATCC 12022 | Good Growth on Subculture Medium | |
| Klebsiella pneumoniae ATCC 13883 | Good Growth on Subculture Medium |
For 1 liter of medium
| Disodium hydrogen phosphate | 1,1 g |
| Sodium thioglycollate | 1,1 g |
| Sodium chloride | 5,0 g |
| Calcium chloride | 0,09 g |
| Agar | 5,6 g |
pH of the ready-to-use medium at 250C: 8,4 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ BacterTube™ Cary Blair | 08001 | 50 tubes/ box or as per customer request. |
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