BacterTube™ Carybac

The medium used for preserving and transporting specimens for anaerobic bacterial culture testing.
Code: 08003
Packaging: 50 tubes/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 6 months from the date of manufacture.

INTENDED USE

BacterTube™ Carybac is a transport and preservation medium designed to maintain the viability of anaerobic bacteria during specimen collection, transportation, and storage. The medium supports the recovery of anaerobic organisms in clinical and research microbiology application.

PRINCIPLES

BacterTube™ Carybac is formulated to provide a reduced environment for anaerobic bacteria, ensuring their survival during transport:
- Tryptone and Yeast Extract: Provide essential nutrients such as amino acids, peptides, and vitamins to maintain bacterial viability.
- Glucose Monohydrate: Acts as a carbohydrate source, supporting metabolic activity.
- Sodium Thioglycollate and L-Cystine: Create a reduced environment by neutralizing oxygen, which is critical for preserving anaerobic conditions.
- Resazurin: Serves as an oxidation-reduction indicator; the medium is pink in the presence of oxygen and colorless in anaerobic conditions.
- Bacteriological Agar: Provides a semi-solid consistency to stabilize specimens during transport.

Use a sterile swab to collect the specimen. Insert the swab into the transport medium up to one-third of the tube’s length, then cut or break off the handle. Secure the cap tightly.
For gastric biopsy samples, press the biopsy fragment down to one-third of the medium’s length in the tube.
Label the tube and send it to the laboratory as soon as possible, maintaining storage conditions at 4°C.
Subculture the specimen onto appropriate culture media.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Escherichia coli ATCC 25922	18 – 24 hours, 35 – 37^oC, 5 – 10% CO₂	Good Growth on Subculture Medium
Staphylococcus aureus ATCC 25923		Good Growth on Subculture Medium
Clostridium perfringens ATCC 13124		Good Growth on Subculture Medium

For 1 liter of medium