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INTENDED USE
- BacterTube™ MKTTn is used to detect selective enrichment and isolation of Salmonella.
PRINCIPLES
- BacterTube™ MKTTn The medium was first developed by Muller and colleagues. It was later improved by Kauffmann by adding bovine bile and brilliant green to enhance selectivity. Finally, Jeffries and colleagues reported the addition of 40 mg/liter of novobiocin to strengthen the inhibition of Proteus species.
- BacterTube™ MKTTn contains nutrient-rich components and selective agents such as casein enzymic hydrolysate and peptic digest of animal tissue, which provide sources of carbon, nitrogen, vitamins, and minerals. Bovine bile and brilliant green are selective agents that inhibit Gram-positive organisms and other Gram-negative organisms. Calcium carbonate acts as a buffering system. Sodium chloride maintains osmotic balance. Sodium thiosulphate is a source of sulfur. Tetrathionate (S4O6) forms selective agents in enrichment media. Novobiocin is added to inhibit Proteus species.
- Transfer 1 mL of the enrichment culture into a prepared MKTTn tube or into a ready-to-melt pre-prepared medium.
- Incubate for 24 ± 3 hours:
- At 36 ± 2 °C to detect Salmonellae in water.
- At 34 to 38 °C to detect Salmonellae in food microbiology methods.
- At 41.5 ± 1 °C to detect Salmonellae in veterinary livestock samples.
RESULTS
- Isolate on XLD agar and on a second selective isolation medium, with a single loop inoculation.
- In the presence of characteristic colonies, perform the necessary confirmatory tests.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference Strains | Incubation conditions | Expected results |
| Salmonella Enteritidis WDCM 00030 | 18 – 24 hours, incubation at 35 – 37oC, Aerobic | > 10 colonies |
| Salmonella Typhimurium WDCM 00031 | > 10 colonies | |
| Escherichia coli WDCM 00013 | ≤ 100 colonies | |
| Enterococcus faecalis WDCM 0008 | <10 colonies |
For 1 liter of medium
| Meat extract | 4,3g |
| Enzymatic digest of casein | 8,6 g |
| Sodium chloride | 2,6 g |
| Calcium carbonate | 38,7 g |
| Sodium thiosulphate (anhydrous) | 30,5 g |
| Ox bile | 4,78 g |
| Brilliant green | 0,0096 g |
pH of the ready-to-use medium at 25°C: 8,0 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ MKTTn | 08049 | 50 tubes/ box or as per customer request. |
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