Vn
BacterTube™ Phenylalanine Deaminase Agar (PAD)
- The medium is used to identify Gram-negative bacilli that are urease-positive.
- Code: 08037
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
INTENDED USE
- BacterTube™ Phenylalanine Deaminase Agar (PAD) is recommended for differentiating Proteus and Providencia species from other members of the Enterobacteriaceae family based on their ability to deaminate phenylalanine to form phenylpyruvic acid.
PRINCIPLES
- Proteus and Providencia species can deaminate phenylalanine to produce phenylpyruvic acid.
- Meat extract provides essential growth nutrients.
- Sodium chloride maintains osmotic balance.
- After incubation, phenylpyruvic acid reacts with ferric chloride to produce a green color, indicating a positive result
- Inoculate the BacterTube™ Phenylalanine Deaminase Agar (PAD) with the cultured bacteria.
- Incubate the inoculated tubes at 35 – 37°C for 24 hours.
- After incubation, add the reagent to the tubes.
RESULTS
- Positive result: The medium turns dark green. This color change is due to degradation products produced by phenylalanine. These products react with the iron compounds in the acidic environment, resulting in the dark green color.
- Negative result: The color of the medium remains unchanged, staying straw yellow (no PPA to react with FeCl3)
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Proteus mirabilis ATCC 12453 | 24 – 48 hours, 35 – 37oC | Color change after adding 10% FeCl₃ solution: Positive result. |
| Escherichia coli ATCC 25922 | No color change after adding 10% FeCl₃ solution: Negative result |
For 1 liter of medium
| Meat Extract | 3,0 g |
| DL-Phenylalanin | 2,0 g |
| Natri clorua | 5,0 g |
| Natri photphate | 1,0 g |
| Agar | 12,0 g |
pH of the ready-to-use medium at 25°C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ Phenylalanine Deaminase Agar (PAD) | 08037 | 50 tubes/ box or as per customer request. |
Related products
BacterContact™ Plate Count Agar
- Ready-to-use medium on 60mm plates for surface microbiological sampling
- Code: 12010
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterBag™ Buffered Peptone Water (BPW)
- The non-selective enrichment medium is used in the pre-enrichment step of the Salmonella testing process.
- Code: 10001
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterStain™ Gram Stain Kit
- A set of reagents used in a technique for the differentiation of Gram-negative and Gram-positive microorganisms.
- Code: 04001
- Packaging: Crystal Violet; Lugol; Safranin; Alcohol: 4 bottles/box 100ml or as per customer request.
- Storage Conditions: 18–25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
BacterTest™ FeCl3
- Reagent is used to perform the Phenylalanie deaminase (PAD) test
- Code: 06002
- Packaging: 100mL/ dropper bottle or as per customer request.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 12 months from the date of manufacture.
BacterPlate™ XLD Agar
- Ready-to-use medium on 90mm plates for detecting Salmonella and Shigella.
- Code: 05043
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
BacterContact™ Pro Tryptic Soy Agar + LT
- Ready-to-use medium on 60mm plates for surface microbiological sampling with the addition of a neutralizer to neutralize surface disinfectants
- Code: 12014
- Packaging: Three-layer packaging irradiated with 10 discs per box, or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 9 months from the date of manufacture.
BacterTube™ Methyl Red-Voges Proskauer (MR-VP)
- Test medium used for the identification of members of the Enterobacteriaceae family
- Code: 08033
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
BacterPlate™ Salmonella Shigella Agar (SS)
- Ready-to-use medium on 90mm plates for the selective culture of Salmonella and Shigella. Applied for culturing stool samples.
- Code: 05019
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterBag™ Dry Half Fraser Broth
- Dry powder form of Half Fraser Broth medium, used for the enrichment of Listeria monocytogenes.
- Code: 10014
- Packaging: Equivalent to 20L of medium per bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 36 months from the date of manufacture.
BacterContact™ Sabouraud Dextrose Agar
- Ready-to-use medium on 60mm plates for the determination of the total count of yeasts and molds.
- Code: 12003
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.