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INTENDED USE
- BacterTube™ Sabouraud Dextrose Broth (SDB) is recommended for the detection of Candida albicans in non-sterile products according to the Pharmacopoeia. It is also used as a nutrient medium for the growth of yeasts and molds. The typical composition meets the requirements of the European and United States Pharmacopoeia.
PRINCIPLES
- BacterTube™ Sabouraud Dextrose Broth (SDB) with ingredients such as meat extract and casein, which provide nitrogen and carbohydrates, it serves as a factor for the growth of yeasts, molds, and acidophilic bacteria. Glucose is the energy source.
- According to the Pharmacopoeia, transfer 1 mL of the sample for testing and its serial dilutions into 10 mL of Sabouraud Dextrose Broth.
- Incubate at 30 – 35°C for 3 to 5 days.
- Subculture onto Sabouraud Dextrose Agar (SDA) and incubate for 24 to 48 hours at 30 – 35°C.
Note:
- Types of yeasts and molds, incubate at 20 – 25°C for 2 to 3 days.
RESULTS
- Observe the results by the growth indicated by the turbidity of the inoculated medium.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Candida albicans ATCC 10231 | 72 hours, 30 – 35oC, Aerobic | Good growth |
| Saccharomyces cerevisiae ATCC 9763 | 48 – 72 hours, 20 – 25oC, Aerobic | Good growth |
| Aspergillus brasiliensis ATCC 16404 | Good growth |
For 1 liter of medium
| Tryptone | 5,0 g |
| Peptic digest of Meat | 5,0 g |
| Dextrose | 20,0 g |
pH of the ready-to-use medium at 25°C: 5,6 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ Sabouraud Dextrose Broth (SDB) | 08051 | 50 tubes/ box or as per customer request. |
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