Blood Agar (BA)
- Transport medium helps preserve bacteria that are difficult to grow, such as Haemophilus influenzae, Streptococcus pneumoniae, and Neisseria species
- Code: 08021
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
INTENDED USE
- BacterTube™ Blood Agar (BA) is primarily used for preserving and transporting bacteria under warm environmental conditions. This medium helps maintain the viability of bacteria for a short period and ensures the survival of hard-to-culture bacteria such as Haemophilus influenzae, Streptococcus pneumoniae, and Neisseria spp. and other bacterial strains that require blood-derived nutrients. Using this medium in slant tubes provides a larger surface area for bacterial growth and facilitates convenient transportation of samples without compromising their quality.
PRINCIPLES
- Peptones included in the composition of the medium favor the excellent growth of colonies.
- Yeast extract is a source of vitamin B complex.
- Starch is a detoxifying agent and also an energy source.
- Defibrinated sheep blood provides X factor (heme), essential for the growth of many fastidious bacteria, and allows the detection of hemolytic reactions.
- Heat the loop’s tip until it becomes red in the flame, then lightly heat the handle of the loop, ensuring the loop is uniformly hot.
- Inoculate the medium by gently placing the loop’s tip on the surface of the slant agar at the bottom of the tube.
- Next, streak in a zigzag pattern from the bottom of the tube to the top along the surface of the slant..
- Place the tube in the incubator under aerobic conditions at 35 – 37°C for 18 – 24 hours.
RESULTS
Observe the bacterial growth.
- Beta hemolysis
- Streptococci belonging to Lancefield group A appear as small, grey colonies, translucid or opaque, surrounded by a zone of beta hemolysis. Other bacteria may present the same type of hemolysis: Listeria, hemolytic Staphylococci, Escherichia coli and Pseudomonas.
- Staphylococci appear as opaque, yellow-gold or white colonies, with or without type β hemolysis zones.
- Listeria present small zones of beta hemolysis.
- Bacillus cereus form a clear zone surrounding the colonies.
- Alpha hemolysis
- Pneumococci appear as flat, shiny, grey and occasionally mucoid colonies surrounded by a zone of narrow, greenish hemolysis referred to as alpha hemolysis.
- CAMP Factor
- Group B Streptococci produce an extracellular, thermoresistant substance (CAMP Factor) which provokes a triangle of total hemolysis in a zone of incomplete staphylococcal hemolysis, at the junction of the two cultures.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
Reference strains | Incubation Conditions | Standard (PR) |
S. pneumonia ATCC 49619 | Incubate for 24 – 48 hours, at 35 – 37°C, with inoculum ≤ 103 – 104 CFU/ mL | Good growth, α hemolysis |
S. pyogenes ATCC 19615 | Good growth, hemolysis | |
E. faecalis ATCC 29212 | Good growth, ɤ hemolysis |
For 1 liter of medium
Polypeptone | 23,0 g |
Starch | 1,0 g |
Sodium chloride | 5,0 g |
Defibrinated sheep blood | 50 – 70 mL |
Agar | 13,5 g |
pH of the ready-to-use medium at 25°C: 7,2 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
Product Name | Code | Packaging |
BacterTube™ Blood Agar (BA) | 08021 | 50 tubes/ box or as per customer request. |
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