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BacterChrom™ StrepB Agar Plates
- Ready-to-use chromogenic medium on 90mm plates for isolating and differentiating Streptococcus group B (S. agalactiae).
- Code: 01003
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ StrepB is a selective chromogenic culture medium that is intended to aid in the qualitative determination of Group B Streptococcus (GBS) colonization in pregnant women. This medium supports the growth of hemolytic and nonhemolytic GBS strains.
- BacterChrom™ StrepB is not intended to diagnose infection nor to guide nor monitor treatment for infections. Further identification, susceptibility testing and epidemiological typing is needed on suspect colonie
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- The medium contains components that facilitate the differentiation of Group B Streptococcus (GBS) through distinctive color changes in the colonies, allowing easy identification of suspected colonies. These components support the growth of both hemolytic and non-hemolytic strains of GBS, ensuring a reliable and accurate detection process.
- BacterChrom™ StrepB can be used with the following specimens: urine, rectal and vaginal specimens. Sampling and transport equipment must be used in accordance with the recommendations of their suppliers for the conservation of Group B Streptococcus.
- Relevant samples can be processed either by direct inoculation onto plates or by pre-enrichment in an enrichment medium (as recommended by the CDC).
- Bring the agar plates to room temperature. Dry the agar plates in an incubator by partially opening the agar plates
- Streak sample onto plate.
- Incubate in aerobic conditions at 35 – 37 °C for 18 – 24 hours
RESULTS
- Qualitative reading and interpretation of the plates
| Microorganisms | Typical colony appearance |
| Streptococcus agalactiae (group B) | mauve |
| Enterococcus species | steel blue |
| Lactobacilli, Leuconostoc, Lactococci | light pink
Scanty growth to inhibited |
| Other microorganism | blue, colourless or inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Strains | Incubation Conditions | Culture results |
| C.albicans ATCC 10321 | Incubate at 35 – 37°C for 24 – 48 hours | Good growth, light pink colonies |
| E.coli ATCC 35218 | Inhibites |
For 1 liter of medium
| Agar | 15,0 g |
| Salts | 7,5 g |
| Peptone and yeast extract | 20,0 g |
| Chromogenic mix | 2,2 g |
| Growth factors mix | 8 mL |
| Selective mix | 0,25g |
pH of the ready-to-use medium at 250C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ StrepB Agar | 01002 | 10 plates/box or as per customer request. |
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