BacterPlate™ Chocolate Agar with VTAP (HP VTAP)

Ready-to-use medium on 90mm plates supplemented with VTAP for the selective culture of Helicobacter pylori
Code: 05011
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 04 months from the date of manufacture.

INTENDED USE

BacterPlate™ Chocolate Agar with VTAP (HP VTAP) is selective medium designed for the cultivation and isolation of Helicobacter pylori from clinical specimens. This medium, supplemented with Vancomycin, Trimethoprim, Amphotericin B, and Polymyxin B, effectively inhibits the growth of contaminants such as Gram-positive bacteria, Gram-negative bacteria, and fungi, making it highly specific for H. pylori. It is ideal for clinical diagnostic applications and microbiological quality control testing.
The typical composition of base medium (Columbia Agar) corresponds to that defined in European (EP), United States (UP) and Japanese (JP) Pharmacopeias.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

Peptones included in the composition of the medium favor the excellent growth of colonies.
Yeast extract is a source of vitamin B complex.
Starch is a detoxifying agent and also an energy source.
The heat treatment of blood in the medium lyses red blood cells, releasing growth factors such as hemin (X factor) and NAD (V factor), which are essential for the growth of certain bacteria.
Vancomycin inhibits Gram-positive bacteria.
Trimethoprim suppresses Gram-negative bacteria by interfering with folate synthesis.
Amphotericin B prevents fungal contamination.
Polymyxin B targets Gram-negative bacteria, enhancing selectivity for pylori.

Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
Inoculate in order to obtain isolated colonies.
Incubate at 37 °C, 5 – 10% CO₂ for 3 to 5 days in optimal conditions for the culture of the inoculated germs

RESULTS

After incubation for the required duration, observe the colonies growing on the surface of the plate for characteristic traits such as colony size, color, and morphology.
- Helicobacter pylori: Small, round, translucent colonies with a smooth appearance, often greyish-white
- Contaminants (Gram-positive, Gram-negative bacteria, or fungi) should be inhibited by the VTAP selective agents.
Identification of the isolated bacteria must be carried out through appropriate subsequent tests

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.