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BacterPlate™ Chocolate Agar with Multivitox
- Medium for culturing fastidious microorganisms, particularly Neisseria and Haemophilus.
- Code: 05017
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Chocolate Agar with Multivitox is an enriched culture medium specifically formulated for the isolation and cultivation of Haemophilus influenzae, Neisseria gonorrhoeae, and other fastidious microorganisms requiring hemin (X factor) and NAD (V factor) for growth. The Multivitox supplement, a proprietary blend of vitamins, cofactors, and detoxifying agents, enhances the growth of these organisms while improving their recovery from clinical specimens. This medium is widely used in clinical diagnostics and microbiological quality control.
- The typical composition of base medium (Columbia Agar) corresponds to that defined in European (EP), United States (UP) and Japanese (JP) Pharmacopeias.
PRINCIPLES
- Peptones included in the composition of the medium favor the excellent growth of colonies.
- Yeast extract is a source of vitamin B complex.
- Starch is a detoxifying agent and also an energy source.
- The heat treatment of blood in the medium lyses red blood cells, releasing growth factors such as hemin (X factor) and NAD (V factor), which are essential for the growth of certain bacteria.
- Multivitox Supplement: A proprietary blend of vitamins and cofactors that promote the growth of fastidious organisms, along with detoxifying agents that neutralize metabolic inhibitors in samples.
- Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
- Inoculate in order to obtain isolated colonies.
- Incubate at 37 °C, 5 – 10% CO2 for 18 – 24 hours in optimal conditions for the culture of the inoculated germs.
RESULTS
- After incubation for the required duration, observe the colonies growing on the surface of the plate for characteristic traits such as colony size, color, and morphology.
- Identification of the isolated bacteria must be carried out through appropriate subsequent tests
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation Conditions | Expected results |
| H. influenzae ATCC 49766 | 35 – 370C, 5 – 10 % CO2, 18 – 24 hours | Good growth |
| S. pneumoniae ATCC 49619 | Weak growth or no growth | |
| Neisseria meningitidis ATCC 13090 | Weak growth or no growth |
For 1 liter of medium
| Polypeptone | 23,0 g |
| Starch | 1,0 g |
| Sodium chloride | 5,0 g |
| Defibrinated horse blood | 50 – 70 mL |
| MultiVitox | 1 set |
| Agar | 13,5 g |
pH of the ready-to-use medium at 250C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Chocolate Agar with Multivitox | 05017 | 10 plates/ box or as per customer request. |
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