BacterPlate™ Chocolate Agar with Multivitox

Medium for culturing fastidious microorganisms, particularly Neisseria and Haemophilus.
Code: 05017
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterPlate™ Chocolate Agar with Multivitox is an enriched culture medium specifically formulated for the isolation and cultivation of Haemophilus influenzae, Neisseria gonorrhoeae, and other fastidious microorganisms requiring hemin (X factor) and NAD (V factor) for growth. The Multivitox supplement, a proprietary blend of vitamins, cofactors, and detoxifying agents, enhances the growth of these organisms while improving their recovery from clinical specimens. This medium is widely used in clinical diagnostics and microbiological quality control.
The typical composition of base medium (Columbia Agar) corresponds to that defined in European (EP), United States (UP) and Japanese (JP) Pharmacopeias.

PRINCIPLES

Peptones included in the composition of the medium favor the excellent growth of colonies.
Yeast extract is a source of vitamin B complex.
Starch is a detoxifying agent and also an energy source.
The heat treatment of blood in the medium lyses red blood cells, releasing growth factors such as hemin (X factor) and NAD (V factor), which are essential for the growth of certain bacteria.
Multivitox Supplement: A proprietary blend of vitamins and cofactors that promote the growth of fastidious organisms, along with detoxifying agents that neutralize metabolic inhibitors in samples.

Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
Inoculate in order to obtain isolated colonies.
Incubate at 37 °C, 5 – 10% CO₂ for 18 – 24 hours in optimal conditions for the culture of the inoculated germs.

RESULTS

After incubation for the required duration, observe the colonies growing on the surface of the plate for characteristic traits such as colony size, color, and morphology.
Identification of the isolated bacteria must be carried out through appropriate subsequent tests

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation Conditions	Expected results
H. influenzae ATCC 49766	35 – 37⁰C, 5 – 10 % CO₂, 18 – 24 hours	Good growth
S. pneumoniae ATCC 49619		Weak growth or no growth
Neisseria meningitidis ATCC 13090		Weak growth or no growth