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BacterChrom™ AOLA Plates
- Ready-to-use chromogenic medium on 90mm plates for the detection, enumeration and isolation of Listeria monocytogenes and Listeria spp.
- Code: 01046
- Packaging: 10 plates/box or as per customer requirem ents.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ AOLA is a selective chromogenic medium designed for the detection, enumeration, and isolation of Listeria monocytogenes and Listeria spp. It is widely used in food safety testing and quality control to detect contamination in raw materials and food products.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ AOLA utilizes a nutrient-rich base and chromogenic substrates to support the growth and differentiation of Listeria spp.:
- Peptones and Yeast Extract: Provide essential nutrients for microbial growth.
- Chromogenic Mix: Enables differentiation of Listeria monocytogenes through blue colonies with a white halo and innocua as blue colonies without a halo.
- Selective Agents: Suppress competing microorganisms, enhancing specificity.
- This medium supports the reliable detection of Listeria spp., facilitating targeted identification and ensuring compliance with food safety standards.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Streak samples directly onto plates
- Incubate in aerobic conditions at 37°C for 24h ± 2h. If suspected colonies of monocytogenes or Listeria spp. are not visible at 24h, continue incubation until 48h ± 2h.
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| L. monocytogenes. | Blue colonies with white halo |
| L. innocua | Blue colonies without white halo |
| Other microorganisms | Blue, colourless or inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| L. monocytogenes ATCC 13932 | Incubate for 24 – 48 hours at 35 – 37°C | Blue colonies with white halo |
| E. faecalis ATCC 29212 | Inhibited | |
| E. coli ATCC 35218 | Inhibited |
For 1 liter of medium
| Peptone and yeast extract | 34 g |
| Salts & growth factors | 21 g |
| Chromogenic mix | 0,6 g |
| Enrichment mix (Supplement E1) | 2,0 g |
| Enrichment mix (Supplement E2) | 7,0 g |
| Selective mix (Supplement S) | 0,08 g |
| Agar | 15,0 g |
pH of the ready-to-use medium at 25°C: 7,2 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ AOLA | 01046 | 10 plates/box or as per customer request. |
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