BacterChrom™ ESBL Agar Plates

Ready-to-use chromogenic medium on 90mm plates for detecting gram-negative bacteria producing Extended-Spectrum Beta–Lactamase.
Code: 01009
Packaging: 10 plates/box or as per customer requirements.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterChrom^TM ESBL Agar is a selective and differential chromogenic culture medium, intended for use in the qualitative direct detection of gastrointestinal colonization with Extended-spectrum-beta-lactamase-resistant Enterobacteria (ESBL) to aid in the prevention and control of ESBL in healthcare settings.
The medium can also be used as an early warning indicator for diagnostic tests of infections to signal the possible presence of multi drug-resistant bacteria.
BacterChrom^TMESBL Agar is not intended to diagnose ESBL infection nor to guide nor monitor treatment for infections.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterChrom^TM ESBL Agar contains a nutrient-rich base, selective agents, and chromogenic substrates for differentiation:Antibiotic mix (e.g., cefpodoxime): Inhibits non-ESBL-producing bacteria while allowing selective growth of ESBL-producing strains.Chromogenic and natural substrates: Facilitate direct identification of common ESBL-producing bacteria:
- Escherichia coli: Pink to reddish colonies (β-glucuronidase activity).
- Klebsiella, Enterobacter, Serratia, Citrobacter (KESC group): Green, olive-brown, or blue colonies (β-glucosidase activity).
- Proteeae (Proteus, Providencia, Morganella): Dark to light brown colonies (deaminase activity).

BacterChrom^TM ESBL Agar can be used with the following specimens: Rectal swab and stools.
Allow plates to reach room temperature before inoculation.
Streak the specimen directly onto the agar surface for isolation.
Incubate in aerobic conditions at 35 – 37 °C for 18 – 24 hours.

RESULTS

Microoragnism	Typical colony appearance
ESBL E. coli	dark pink to reddish
ESBL KEC (Klebsiella, Enterobacter, Citrobacter)	metallic blue (+/- reddish halo)
ESBL Proteus	brown halo
ESBL Acinetobacter	cream
ESBL Pseudomonas	translucent, (+/- natural pigmentation cream to green)
Stenotrophomonas	colourless
Gram (+) strains	inhibited
Non Resistant Other Gram (-) strains	inhibited
Yeasts	mostly inhibited

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
ESBL E.coli CIP 103982	Incubate for 16 – 24 hours at 35 – 37 °C, 5% CO₂	Good growth, small reddish colonies
ESBL K. Pneumoniae ATCC 700603		Good growth, blue colonies
E.faecalis ATCC 29212		Inhibited