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BacterChrom™ Streptococcus Plates
- Ready-to-use chromogenic medium on 90mm plates for the isolation and differentiation of Streptococcus spp.
- Code: 01037
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ Streptococcus is a selective chromogenic medium designed for the isolation and differentiation of Streptococcus species. It is particularly useful in detecting environmental Streptococci, which are major mastitis pathogens causing persistent infections, high somatic cell counts, and milk contamination.
- The medium supports mastitis diagnosis by enabling differentiation of species involved in udder infections. It is also effective in samples with complex bacterial flora, such as milk, where Streptococci are prevalent.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ Streptococcus utilizes chromogenic substrates that react with specific enzymatic activities of target bacteria, resulting in colonies with distinct colors for differentiation. The medium also incorporates selective agents to inhibit the growth of non-target organisms, ensuring high specificity. This combination simplifies and accelerates the identification process, supporting accurate and efficient diagnostics.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Direct streaking sample onto the plate
- Incubate in aerobic conditions at 35 – 37°C and read at 24h.
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| S. agalactiae | blue |
| S. uberis | mostly pink to purple |
| Enterococcus | mauve to violet-mauve |
| Other Streptococcus | various |
| Other Gram (+) bacteria | inhibited |
| Gram (-) bacteria | inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| S. agalactiae ATCC 12386 | Incubate for 24 hours at 35 – 37 °C | Blue colonies |
| E. faecalis ATCC 29212 | Mauve colonies | |
| C. albicans ATCC 10231 | Inhibited | |
| E. coli ATCC 35218 | Inhibited | |
| S. aureus ATCC 25923 | Inhibited |
For 1 liter of medium
| Peptone and yeast | 8,3 g |
| Chromogenic mix | 20,4 g |
| Agar | 15,0 g |
| Salt | 7,5 g |
| Growth factor | 8 mL |
pH of the ready-to-use medium at 25°C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ Streptococcus | 01037 | 10 plates/box or as per customer request. |
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