BacterChrom™ UTI Agar Plates

Ready-to-use chromogenic medium on 90mm plates for isolating and differentiating urinary tract pathogenic microorganisms.
Code: 01012
Packaging: 10 plates/box or as per customer requirements.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterChrom™ UTI Agar is a chromogenic medium for the presumptive identification and differentiation of all the main micro-organisms that cause urinary tract infections (UTIs).
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterChrom™ UTI Agar contains two specific chromogenic substrates which are cleaved by enzymes produced by Enterococcus spp., Escherichia coli and coliforms. In addition, it contains phenylalanine and tryptophan, which provide an indication of tryptophan deaminase activity, indicating the presence of Proteus spp., Morganella spp. and Providencia spp. It is based on electrolyte deficient CLED Medium which provides a valuable non-inhibitory diagnostic agar for plate culture of other urinary organisms, whilst preventing the swarming of Proteus spp.
One chromogen, X-Gluc, is targeted towards β-glucosidase, and allows the specific detection of enterococci through the formation of blue colonies. The other chromogen, Red-Gal, is cleaved by the enzyme β-galactosidase which is produced by Escherichia coli, resulting in pink colonies. Any uncertainty in identification may be resolved by removing suspect Escherichia coli colonies from the plate and performing an indole test using DMACA reagent. Cleavage of both chromogens occurs in the presence of coliforms, resulting in purple colonies.
The medium also contains tryptophan which acts as an indicator of tryptophan deaminase activity, resulting in colonies of Proteus, Morganella and Providencia spp. appearing brown.

Bring the agar plates to room temperature. Dry the agar plates in an incubator by partially opening the agar plates.
Inoculate samples from the enrichment medium or direct samples onto the surface of the agar plates.
Incubate the inoculated plates under aerobic conditions at 35 – 37 0C, for 18 to 24 hours

RESULTS

Bacterium	ß-galactosidase	ß-glucosidase	TDA	Color of colonies
Enterococci	–	+	–	Blue
Escherichia coli	+	–	–	Pink
Coliforms	+	+	–	Purple
Proteus/Morganella & Providencia spp.	–	–	+	Brown
Pseudomonads	–	–	–	Green or fluorescent brown
Staphylococci	–	–	–	Natural pigment:

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Strains	Incubation Conditions	Culture results
E. coli ATCC 25922	Incubate at 35 – 37°C for 18 – 24 hours	Good growth, pink colonies
S.aureus ATCC 25923		Good growth, light yellow colonies
E. faecalis ATCC 29212		Good growth, blue colonies