Vn
BacterPlate™ Bile Esculin Agar (BEA)
- Ready-to-use medium on 90mm plate used for the isolation and enumeration of enterococci in food and pharmaceutical products.
- Code: 05059
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Bile Esculin Agar (BEA) is a selective medium used for the isolation and enumeration of Enterococci in food and pharmaceutical products. In addition, the medium is also used for the enumeration of Enterococci in animal feed.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterPlate™ Bile Esculin Agar (BEA) contains esculin, ferric citrate to provide ferric ions, and 4% oxbile to inhibit most other strains of nongroup D streptococci. Esculin is hydrolyzed by group D streptococci to form dextrose and esculetin. This compound reacts with the ferric ions contained within the medium, turning the medium from its original amber color to a dark brown to black. Thus the tolerance to the presence of oxbile and the hydrolysis of esculin provide the means to presumptively identify group D streptococci
- Allow thep plate warm to room temperature before use.
- Inoculate and streak the medium with one isolated pure colony.
- Incubate in an aerobic atmosphere at 35ºC for 24 – 48 hours.
- Observe for growth and blackening of the medium.
NOTE:
- This product is not intended for primary isolation of patient specimens. It should be used only with cultures of isolated organism.
This product is used in conjunction with other biochemical tests to identify cultures of isolated organism.
RESULTS
- The enterococci appear as small translucent colonies surrounded by a black halo.
- Staphylococci and yeasts may yield opaque colonies without a black halo.
- It is indispensable to identify suspected bacteria, especially to eliminate confusion with Listeria which may give rise to colonies similar to those of enterococci.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference Strains | Incubation Conditions | Expected Results |
| E. faecalis ATCC 29212 | 35 – 37°C
18 – 24 hours, aerobic |
Good growth, black zone around the colony |
| E. coli ATCC 35218 | Inhibited, no growth |
For 1 liter of medium
| Tryptone | 17,0 g |
| Peptic digest of meat | 3,0 g |
| Yeast extract | 5,0 g |
| Bacteriological ox bile | 10,0 g |
| Sodium chloride | 5,0 g |
| Esculin | 1,0 g |
| Ferric ammonium citrate | 0,5 g |
| Sodium azide | 0,15 g |
| Agar | 13,0 g |
pH of the ready-to-use medium at 25°C: 7,1 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Bile Esculin Agar (BEA) | 05059 | 10 plates/ box or as per customer request. |
Related products
BacterPlate™ Chocolate Agar with VTCN (HP VTCN)
- Ready-to-use medium on 90mm plates supplemented with VTCN for the selective culture of Helicobacter pylori
- Code: 05012
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterChrom™ Mastitis Plates
- Ready-to-use chromogenic medium on 90mm plates for the isolation and differentiation of the main pathogens involved in Mastitis infections
- Code: 01039
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterPlate™ Chocolate Agar with VCN (CA VCN)
- Ready-to-use medium on 90mm plates for the selective culture of Neisseria gonorrhoeae and Neisseria meningitidis.
- Code: 05010
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
BacterChrom™ Cronobacter Plates
- Ready-to-use chromogenic medium on 90mm plates for isolating Cronobacter bacteria according to ISO 22964 standards.
- Code: 01043
- Packaging: 10 plates/box or as per customer requirem ents.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
Vertical Laminar Flow Cabinet, HUYAir VLF
The HUYAir VLF is a series of highly efficient Vertical Laminar Flow (VLF) Microbiological Safety Cabinets designed to protect samples, equipment, and other components in the work area from molecular to highly sensitive applications. It is ideal for use with non-hazardous and easily accessible materials in the work area as desired. The key feature of the VLF cabinets is to create a clean working environment for a wide range of applications and utilize UV lamps for sterilization, cleaning the work area, and internal components during operation with the LCD digital control system.
- Complies with standards: NSF 49, EN 12469.
- The cabinet is certified by the National Institute of Hygiene and Epidemiology.
- Size standard: 900mm | 1200mm | 1500mm | 1800mm
- Warranty: 03 years.
Class I Biological Safety Cabinet, HUYAir Bio1
Class I Biological Safety Cabinet, HUYAir Bio1 is designed to provide safety protection for operators and the environment during direct handling of contaminated samples with a high potential for disease transmission. The Class 1 cabinet is a cost-effective alternative to Class 2 biological safety cabinets, suitable for applications that do not require product protection.
- Standard compliance: NSF 49, EN 12469.
- The cabinet meets the Class 1 Biological Safety Cabinet standards issued by the National Institute of Hygiene and Epidemiology.
- Size standard: 900mm | 1200mm | 1500mm | 1800mm.
- Warranty: 3 years.
BacterPlate™ Chocolate Agar with Multivitox
- Medium for culturing fastidious microorganisms, particularly Neisseria and Haemophilus.
- Code: 05017
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterChrom™ Enterobacteria Plates
- Ready-to-use chromogenic medium on 90mm plates for the detection and enumeration of Enterobacteriaceae
- Code: 01033
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterChrom™ Pseudomonas Plates
- Ready-to-use chromogenic medium on 90mm plates for the detection and isolation of Pseudomonas spp.
- Code: 01042
- Packaging: 10 plates/box or as per customer requirem ents.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterPlate™ Blood Agar
- Ready-to-use medium on 90mm plates for culturing and differentiating hemolytic phenotypes α, β, and ɤ.
- Code: 05004
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.