BacterPlate™ Horse Blood Agar (HBA)

Ready-to-use medium on 90mm plates used for the non-selective culture of fastidious bacteria (Haemophilus, Neisseria spp).
Code: 05007
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 04 months from the date of manufacture.

INTENDED USE

BacterPlate™ Horse Blood Agar (HBA) is an enriched, non-selective medium used for the isolation, cultivation, and differentiation of fastidious microorganisms, including Streptococcus spp. and Haemophilus spp.
The typical composition of base medium (Columbia Agar) corresponds to that defined in European (EP), United States (UP) and Japanese (JP) Pharmacopeias.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

Peptones included in the composition of the medium favor the excellent growth of colonies.
Yeast extract is a source of vitamin B complex.
Starch is a detoxifying agent and also an energy source.
The addition of sterile defibrinated horse blood enables the observation of hemolytic activity.

Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
Inoculate in order to obtain isolated colonies.
Incubate at 37°C, 5 – 10% CO₂ for 24 to 48 hours in optimal conditions for the culture of the inoculated germs.

RESULTS

After incubation for the required duration, observe the colonies growing on the surface of the plate for characteristic traits such as colony size, color, and morphology.
Helicobacter pylori: Small, round, translucent colonies with a smooth appearance, often greyish-white
Contaminants (Gram-positive, Gram-negative bacteria, or fungi) should be inhibited by the VTAP selective agents.
Identification of the isolated bacteria must be carried out through appropriate subsequent tests

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Microbial Standard and Incubation Conditions	Standard (P_R)
Incubation conditions after 24 – 48 hours, at 35 – 37°C, 5 – 10% CO2 with inoculum ≤ 10³ – 10⁴ CFU/ mL
H. influenzae ATCC 33533	Good growth
S. pyogenes ATCC 19615	Good growth,