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BacterPlate™ TCBS Agar
- Ready-to-use medium on 90mm plates for isolating and selecting Vibrio. Differentiates Vibrio cholerae from other Vibrio species based on sucrose fermentation capability.
- Code: 05022
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterPlate™ TCBS Agar is a selective medium for the isolation of Vibrio cholerae and other enteropathologic Vibrio (in particular Vibrio parahaemolyticus) in fish, seafood and biological samples of animal origin.
- The typical composition corresponds to that defined in the standard NF EN ISO 21872-1.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLE
- The high concentrations of thiosulfate and sodium citrate, as well as the alkalinity of the medium, considerably inhibit the growth of enterobacteria.
- Ox bile and sodium cholate slow the growth of enterococci and inhibit the development of Gram positive bacteria.
- The acidification of the medium resulting from the fermentation of sucrose by Vibrio makes bromothymol blue turns yellow.
- Using thiosulfate as a sulfur source, the production of hydrogen sulfide is visualized in the presence of ferric citrate. All Vibrio are H2S-negative.
- Inoculate and isolate a loop of each enrichment broth onto the surface of BacterPlate™ TCBS Agar medium and onto a second media of choice.
- Incubate at 36 ± 1 °C for 24 ± 3 hours.
RESULTS
- Colonies present the following aspects
| Micoorganisms | Characteristics |
| Vibrio cholerae
Vibrio fluvialis Vibrio furnissii |
Flat yellow colonies, 2 to 3 mm in diameter |
| Vibrio vulnificus
Vibrio parahaemolyticus Vibrio mimicus |
Flat, green colonies, 2 to 3 mm in diameter |
| Pseudomonas, Aeromonas
Enterobacteriaceae or others |
Blue colonies
Tiny, transparent colonies |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strain | Incubation conditions | Expected result |
| V. parahaemolyticus ATCC 17802 | 18 – 24 hours at 35 – 370C | Good growth, green colonies |
| E. coli ATCC 35218 | Inhibited, no growth |
For 1 liter of medium
| Polypeptone | 10,0 g |
| Yeast extract | 5,0 g |
| Saccharose | 20,0 g |
| Bacteriological ox bile | 5,0 g |
| Sodium cholate | 3,0 g |
| Sodium citrate | 10,0 g |
| Sodium thiosulfate | 10,0 g |
| Sodium chloride | 10,0 g |
| Ferric ammonium citrate | 1,0 g |
| Bromothymol blue | 40,0 mg |
| Thymol blue | 40,0 mg |
| Agar | 14,0 g |
pH of the ready-to-use medium at 250C: 8,6 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ TCBS Agar | 05022 | 10 plates/ box or as per customer request. |
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