BacterPlate™ TCBS Agar

Ready-to-use medium on 90mm plates for isolating and selecting Vibrio. Differentiates Vibrio cholerae from other Vibrio species based on sucrose fermentation capability.
Code: 05022
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterPlate™ TCBS Agar is a selective medium for the isolation of Vibrio cholerae and other enteropathologic Vibrio (in particular Vibrio parahaemolyticus) in fish, seafood and biological samples of animal origin.
The typical composition corresponds to that defined in the standard NF EN ISO 21872-1.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLE

The high concentrations of thiosulfate and sodium citrate, as well as the alkalinity of the medium, considerably inhibit the growth of enterobacteria.
Ox bile and sodium cholate slow the growth of enterococci and inhibit the development of Gram positive bacteria.
The acidification of the medium resulting from the fermentation of sucrose by Vibrio makes bromothymol blue turns yellow.
Using thiosulfate as a sulfur source, the production of hydrogen sulfide is visualized in the presence of ferric citrate. All Vibrio are H₂S-negative.

Inoculate and isolate a loop of each enrichment broth onto the surface of BacterPlate™ TCBS Agar medium and onto a second media of choice.
Incubate at 36 ± 1 °C for 24 ± 3 hours.

RESULTS

Micoorganisms	Characteristics
Vibrio cholerae Vibrio fluvialis Vibrio furnissii	Flat yellow colonies, 2 to 3 mm in diameter
Vibrio vulnificus Vibrio parahaemolyticus Vibrio mimicus	Flat, green colonies, 2 to 3 mm in diameter
Pseudomonas, Aeromonas Enterobacteriaceae or others	Blue colonies Tiny, transparent colonies

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

For 1 liter of medium