BacterPlate™ Two-Compartment Medium ESBL/KPC

Ready-to-use medium on 90mm plates for the detection and differentiation of ESBL producing bacteria and Carbapenemase-producing bacteria (KPC)
Code: 05051
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 04 months from the date of manufacture.

INTENDED USE

BacterPlate™ Two-Compartment Medium ESBL / KPC is a ready-to-use two-compartment agar medium designed for the detection and differentiation of Extended-Spectrum Beta-Lactamase (ESBL) and Carbapenemase (KPC) producing bacteria. This product is used in microbiological research, clinical diagnostics, and microbiological quality control of food, water, pharmaceuticals, and animal-derived biological samples.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterPlate™ Two-Compartment Medium ESBL/KPC

ESBL Compartment: This medium contains selective components such as cefotaxime or ceftazidime, which are used to isolate Enterobacteriaceae strains that produce ESBL. A color indicator is also included to differentiate between ESBL (+) and ESBL (-) colonies.
KPC Compartment: This is a selective medium containing antibiotics such as imipenem or meropenem, used to isolate Carbapenem-resistant Enterobacteriaceae (CRE) strains that produce Carbapenemase (KPC). KPC-producing colonies will grow well despite the presence of antibiotics, while non-Carbapenemase-producing bacteria will either not grow or grow weakly.

Allow the agar plates to reach room temperature before use.
Dry the agar plates in an incubator with the lid slightly open.
Inoculate the sample by spreading it evenly on the surface of the agar using a sterile inoculating loop.
Incubate at 35–37°C for 18–24 hours.

RESULTS

MRSA Compartment

MRSA (+): Colonies have a characteristic color (usually pink/purple/red), growing well.
MRSA (–): No growth or weak growth. (MSSA)

KPC Compartment

KPC (+): Colonies have a characteristic color, growing well despite the presence of antibiotics.
KPC (–): No growth or weak growth.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
KPC Compartment
E.coli IMP NCTC 13476	Incubate at 35–37°C for 18–24 hours.	Good growth, dark pink colonies
K.pneumoniae ATCC BAA 1705		Good growth, metallic blue colonies
K.pneumoniae ATCC 13883		Inhibited
S. aureus ATCC 25293		Inhibited
E.faecalis ATCC 29212		Inhibited
ESBL Compartment
ESBL E.coli CIP 103982	Incubate at 35–37°C for 18–24 hours.	Good growth, small reddish colonies
ESBL K. Pneumoniae ATCC 700603		Good growth, blue colonies
E.faecalis ATCC 29212		Inhibited