BacterPlate™ Two-Compartment Medium MC/SS

Ready-to-use medium on 90mm plates for the cultivation and isolation of infectious agents from fecal, blood, and food samples.
Code: 05047
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 04 months from the date of manufacture.

INTENDED USE

BacterPlate™ Two-Compartment Medium MC/SS combines MacConkey Agar (MC) and Salmonella Shigella Agar (SS), and is used for the isolation and differentiation of enteric bacteria, particularly pathogens such as Salmonella and Shigella from fecal samples. This medium supports the isolation of Gram-negative bacteria, particularly in applications for testing water, food, and pharmaceuticals.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterPlate™ Two-Compartment Medium MC/SS:

MC (MacConkey Agar) Compartment: MacConkey Agar is a selective medium used to isolate Gram-negative bacteria, particularly enteric bacteria. Bacteria capable of lactose fermentation will form red or pink colonies, while non-lactose fermenters will form colorless or transparent colonies.
SS (Salmonella Shigella Agar) Compartment: This medium supports the growth of Salmonella and Shigella while inhibiting the growth of other bacteria such as coliforms. Bacteria that produce hydrogen sulfide (H₂S) form colonies with black centers, while non-lactose fermenters form colorless colonies.

Allow the agar plates to reach room temperature before use.
Dry the agar plates in an incubator with the lid slightly open.
Inoculate the sample by spreading it evenly on the surface of the agar using a sterile inoculating loop.
Incubate at 35 – 37°C for 18–24 hours.

RESULTS

MC Compartment:

Lactose Positive: Lactose-fermenting bacteria will form red colonies, surrounded by a precipitate of bile salts.
Lactose Negative: Non-lactose fermenting bacteria will form colorless or transparent colonies.

SS Compartment:

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
MC Compartment
E. coli ATCC 35218	Incubate at 35–37°C for 18–24 hours.	Good growth
S. aureus ATCC 25923		Weak growth
S. flexneri ATCC 12022		Inhibited, no growth
SS Compartment
S. typhimurium ATCC 14028	Incubate at 35–37°C for 18–24 hours.	Good growth, colorless colonies with black centers
S. flexneri ATCC 12022		Good growth, colorless colonies
E. coli ATCC 35218		Partial inhibition, pink colonies
S. aureus ATCC 25923		Inhibited, no growth

For 1 liter of MC medium

Pancreatic digest of gelatin	17,0 g
Tryptone	1,5 g
Peptic digest of meat	1,5 g
Lactose	10,0 g
Bile salts	1,5 g
Sodium chloride	5,0 g
Neutral red	30,0 mg
Crystal violet	1,0 mg
Agar	13,5 g

pH of the ready-to-use medium at 25°C: 7,1 ± 0,2

For 1 liter of SS medium