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INTENDED USE
- BacterTube™ Alkaline Peptone is used as a primary and secondary enrichment medium for the detection of Vibrio spp., including Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio vulnificus, from food products (human and animal) and environmental samples. The medium complies with the food microbiology standard NF EN ISO 21872-1.
PRINCIPLES
- BacterTube™ Alkaline Peptone creates a high pH (8,6 ± 0,2) and saline environment that favors the growth of Vibrio spp. while inhibiting competing bacteria. Peptone provides essential nutrients, and sodium chloride mimics marine conditions, enhancing the recovery of halophilic Vibrio spp.. The two-step enrichment process increases the concentration of target organisms for easier detection in food and environmental samples.
Detection of Vibrio parahaemolyticus and Vibrio cholerae
- Aseptically add X g of the sample to 9X mL of BacterTube™ Alkaline Peptone, mix thoroughly.
- Incubate for 6 ± 1 hours at:
- 37 ± 1°C for deep-frozen, dried, or salted products.
- 5 ± 1.0°C for fresh products.
- Transfer 1 mL of the culture into 10 mL of fresh BacterTube™ Alkaline Peptone.
- Incubate for 18 ± 1 hours at:
- 5 ± 1.0°C for Vibrio parahaemolyticus and Vibrio cholerae enrichment.
- 37 ± 1°C for Vibrio vulnificus
Note: For general Vibrio spp. detection, all enrichments should be incubated at 37°C.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Vibrio parahaemolyticus ATCC 17802 | 18 – 24 hours, 35 – 37°C | Turbid growth |
| Vibrio vulnificus ATCC 29307 | Turbid growth | |
| Vibrio furnissii ATCC 11218 | Turbid growth |
For 1 liter of medium
| Tryptone | 5,0 g |
| Lactose | 4,0 g |
| Disodium phosphate | 10,0 g |
| L-Cystine | 0,01 g |
| Sodium biselenite | 4,0 g |
pH of the ready-to-use medium at 25°C: 8,6 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ Alkaline Peptone | 08006 | 50 tubes/ box or as per customer request. |
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