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INTENDED USE
- BacterTube™ Urea Broth is recommended for the identification of bacteria based on urea utilization, particularly for differentiating Proteus, Salmonella, and Shigella species from clinical and non-clinical specimens.
PRINCIPLES
- This medium differentiates bacteria based on their ability to hydrolyze urea using the enzyme urease:
- Urea hydrolysis releases ammonia, increasing the medium’s pH, which changes the indicator color to pink/red.
- The high buffering capacity and minimal nutrients prevent false positives caused by protein hydrolysis.
- A negative control without urea ensures accurate results by ruling out false positive
- Prior to inoculation, allow medium to equilibrate to room temperature
- Use a sterile inoculation loop to transfer a sample from an isolated colony on the agar plate into the test tube containing the medium.
- Incubate the tube at 35°C.
- Examine the tube after 6 – 8 hours and again after 12 – 18 hours of incubation.
- Observe and record the results
RESULTS
- Positive Reaction: Pink to red color (urease activity detected).
- Negative Reaction: No color change (yellowish-orange remains)
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Klebsiella pneumoniae ATCC 13883 | 18 – 24 hours, 35 – 37oC | Pink medium: Urease positive |
| Escherichia coli ATCC 25992 | Unchanged medium: Urease negative |
For 1 liter of medium
| Peptone | 1,0 g |
| Glucose | 1,0 g |
| Sodium chloride | 5,0 g |
| Disodium phosphate | 1,2 g |
| Potassium dihydrogen phosphate | 0,8 g |
| Phenol red | 0,004 g |
| Urea | 20,0 g |
pH of the ready-to-use medium at 25°C: 6,8 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ Urea Broth | 08036 | 50 tubes/ box or as per customer request. |
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