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BacterPlate™ Two-Compartment Medium MC/SDA
- Ready-to-use medium on 90mm plates for the cultivation and isolation of Gram-negative bacilli and yeasts from clinical specimens
- Code: 05046
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Two-Compartment Medium MC/SDA combines MacConkey Agar and Sabouraud Dextrose Agar (SDA), and is used for the cultivation and isolation of pathogenic bacteria, as well as yeasts and molds, from clinical specimens. This medium is particularly useful for differentiation.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage
PRINCIPLES
BacterPlate™ Two-Compartment Medium MC/SDA
- MC (MacConkey Agar) Compartment: MacConkey Agar is a selective medium used to isolate Gram-negative bacteria, particularly enteric bacteria. Bacteria capable of lactose fermentation will form red or pink colonies, while non-lactose fermenters will form colorless or transparent colonies.
- SDA (Sabouraud Dextrose Agar) Compartment: The Sabouraud Dextrose Agar (SDA) supports the growth of yeasts and molds. The medium has a low pH, which helps inhibit bacterial growth and promotes the growth of fungal species such as Candida albicans.
- Dry the agar plates in an incubator with the lid slightly open.
- Inoculate the sample by spreading it evenly on the surface of the agar using a sterile inoculating loop.
- Incubate at 35 – 37°C for 18 – 24 hours for bacteria, or at 20 – 25°C for 5 – 7 days for yeasts and molds
RESULTS
MC Compartment:
- Lactose Positive: Lactose-fermenting bacteria will form red colonies, surrounded by a precipitate of bile salts.
- Lactose Negative: Non-lactose fermenting bacteria will form colorless or transparent colonies.
SDA Compartment:
- Candida albicans: Cream-colored colonies, good growth
- Aspergillus brasiliensis: Development of white mycelium with black spores.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| MC Compartment | ||
| E. coli ATCC 35218 | Incubate at 35–37°C for 18–24 hours. | Good growth |
| S. aureus ATCC 25923 | Weak growth | |
| S. flexneri ATCC 12022 | Inhibited, no growth | |
| SDA Compartment | ||
| C. albicans ATCC 10231 | Incubate at 20 – 25°C for 3 – 5 days. | Good growth |
| S. cerevisiae ATCC 20827 | Good growth | |
| A. brasiliensis ATCC 16404 | Good growth | |
For 1 liter of MC medium
| Pancreatic digest of gelatin | 17,0 g |
| Tryptone | 1,5 g |
| Peptic digest of meat | 1,5 g |
| Lactose | 10,0 g |
| Bile salts | 1,5 g |
| Sodium chloride | 5,0 g |
| Neutral red | 30,0 mg |
| Crystal violet | 1,0 mg |
| Agar | 13,5 g |
pH of the ready-to-use medium at 25°C: 7,1 ± 0,2
For 1 liter of SDA medium
| Dextrose | 40,0 g |
| Pancreatic digest of animal tissues | 5,0 g |
| Pancreatic digest of casein | 5,0 g |
| Agar | 15,0 g |
pH of the ready-to-use medium at 25°C: 5,6 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ Two-Compartment Medium MC/SDA | 05046 | 10 plates/ box or as per customer request. |
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