Vn
INTENDED USE
- BacterPlate™ XLD Agar is used for the isolation of Salmonella in pharmaceutical products. It can also be used as a second media of choice in the normalized methods for the detection of Salmonella in food products and water.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLE
- Sodium desoxycholate inhibits contaminating Gram-positive flora. Xylose is fermented by practically all enteropathogenic bacteria, except for Shigella which are thus differentiated from the other species. After exhausting xylose, Salmonella decarboxylate lysine (via lysine decarboxylase) to cadaverine, causing the pH to rise. Colonies of salmonellae resemble those of shigellae in the medium having become basic. The colonies formed are red in the presence of the indicator, phenol red.
- The addition of lactose and sucrose to the medium enable coliform bacteria to decarboxylate lysine and thereby produce excess acidity, making the indicator turn yellow, favoring their differentiation.
- In basic medium, pathogenic H2S-producers reduce ferric ammonium citrate and cause a blackening due to the production of iron sulfide at the center of the colonies. Non-pathogenic bacteria which do not decarboxylate lysine acidify the medium, a result of sugar fermentation. The pH decrease prevents the colonies from blackening.
- Re-inoculate a loop of enrichment media onto BacterPlate™ XLD Agar
- Incubate at 30 – 35 °C for 18 to 48 hours.
Note: For using XLD Agar as the second Salmonella isolation media, incubate the plates 24 hours at 37 °C.
RESULTS
- Salmonella present red colonies, with or without black centers.
- The aspect of the colonies is as follows:
| Microorganisms | Characteristics |
| Escherichia coli, Citrobacter, Enterobacter, Proteus, Serratia, Klebsiella | Yellow colonies, with or without a black center |
| Shigella, Providencia, Salmonella Paratyphi A | Red colonies without black center |
| Salmonella, Edwardsiella | Red colonies with black center |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation Conditions | Expected results |
| E. faecalis ATCC 29212 | 35 ± 2oC for 18 – 24 hours | Inhibited, no growth |
| E.coli ATCC 35218 | Weak growth, yellow colonies | |
| S. Enteritidis ATCC 13076 | Good growth, red colonies with black centers |
For 1 liter of medium
| Yeast extract | 3,0 g |
| L-Lysine hydrochloride | 5,0 g |
| Lactose | 7,5 g |
| Sucrose | 7,5 g |
| Xylose | 3,75 g |
| Sodium desoxycholate | 1,0 g |
| Sodium chloride | 5,0 g |
| Sodium thiosulfate | 6,8 g |
| Ferric ammonium citrate | 0,8 g |
| Phenol red | 0,08 g |
| Agar | 12,5 g |
pH of the ready-to-use medium at 250C: 7,4 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ XLD Agar | 05043 | 10 plates/ box or as per customer request. |
Related products
BacterPlate™ Mueller Hinton + Horse Blood + NAD (MHF)
- Ready-to-use medium on 90mm plates for testing antibiotic susceptibility of difficult-to-cultivate microorganisms, requiring a high concentration of NAD+.
- Code: 05029
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterPlate™ Two-Compartment Medium TCBS/SS
- Ready-to-use medium on 90mm plates for the cultivation and isolation of Vibrio, Salmonella, and Shigella from clinical and research samples.
- Code: 05048
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
BacterPlate™ GC Agar có IsoVitalex
- Ready-to-use medium on 90mm plates for testing antibiotic susceptibility of Neisseria gonorrhoeae bacteria.
- Product code: 05060
- Order code: 060015454
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterPlate™ Sabouraud Dextrose Agar (SDA)
- Ready-to-use medium on 90mm plates for culturing, differentiating, and quantifying yeasts and molds.
- Code: 05027
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
BacterPlate™ Tryptic Soy Agar (TSA)
- Ready-to-use medium on 90mm plates for non-selective culture of easily cultured bacteria.
- Code: 05026
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
BacterPlate™ Horse Blood Agar (HBA)
- Ready-to-use medium on 90mm plates used for the non-selective culture of fastidious bacteria (Haemophilus, Neisseria spp).
- Code: 05007
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
BacterChrom™ MRSA Agar Plates
- Ready-to-use chromogenic medium on 90mm plates for detecting and differentiating MRSA (Methicillin-Resistant Staphylococcus aureus).
- Code: 01048
- Packaging: 10 plates/box or as per customer requirem ents.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterPlate™ Bromocresol Purple Agar (BCP)
- Ready-to-use medium on 90mm plates for differentiating between Enterobacteriaceae and Coliforms
- Code: 05032
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterChrom™ UTI Agar Plates
- Ready-to-use chromogenic medium on 90mm plates for isolating and differentiating urinary tract pathogenic microorganisms.
- Code: 01012
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
BacterPlate™ SDA with Chloramphenicol
- Ready-to-use medium on 90mm plates for selective fungal cultivation. Applied for the selective isolation of fungi from clinical specimens.
- Code: 05028
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.