Vn
INTENDED USE
- TraceMedia™ Sheep Blood Agar is a medium used for cultivation and isolation of various microorganisms.
- It is also used to differentiate bacterial hemolysis types: alpha, beta, and gamma.
- The medium is employed for culturing and isolating infectious agents from respiratory specimens, throat swabs, nasal secretions, etc.
PRINCIPLE
- TraceMedia™ Sheep Blood Agar consists of Columbia Blood Agar Base supplemented with sheep blood, creating a medium that provides growth factors and allows for the determination of bacterial hemolytic reactions.
- On the surface of the agar plate, bacteria with a clear zone of hemolysis are classified as beta hemolysis.
- Bacteria that partially degrade hemoglobin, creating a greenish zone around the colonies, are classified as alpha hemolysis.
- Gamma hemolysis refers to no hemolysis observed on the surface.
READING THE RESULTS
- After incubation for the required time, observe the growth of colonies on the agar plate and their characteristics.
- Further identification of isolated bacteria must be conducted through appropriate tests.
- Samples related will be directly inoculated onto the agar plate.
- If the agar plate has been refrigerated, allow it to warm up to room temperature before inoculating the samples.
- Inoculate the samples onto the agar plate.
- Incubate the samples at 35 – 37°C, 5% CO2 for 16 – 24 hours.
BacterLab checks the quality of each product batch using standard strains from ATCC:
| Microorganism strain | Cultivation Reaction after 16-24 hours | Cultivation result at 35 – 37°C |
| Streptococcus pneumonia ATCC 49619 | Good growth, alpha-hemolytic bacteria | |
| Streptococcus pyogenes ATCC 19615 | Good growth, beta-hemolytic bacteria | |
| Enterococcus faecalis ATCC 29212 | Good growth, non-hemolytic bacteria |
Ingredients per 1 liter (Reference):
| Special peptone | 23 g |
| Starch | 1 g |
| Sodium chloride | 5 g |
| Agar | 10 g |
| Sheep blood | 5 – 7% |
| pH of the complete medium at 25°C: 7.3 ± 0.2 | |
*Please note that this composition is for reference purposes. Additionally, your company will prepare the components as required or according to the tender documents.
| Product name | Product code | Paking |
| TraceMedia™ Sheep Blood Agar | TM0722 | 10 Plates/Box |
Related products
BacterBag™ Maximum Recovery Diluent
- Protective and isotonic medium used for maximal recovery of microorganisms from a variety of sources.
- Code: 10004
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterContact™ Plate Count Agar
- Ready-to-use medium on 60mm plates for surface microbiological sampling
- Code: 12010
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterPlate™ Chocolate Agar with VTAP (HP VTAP)
- Ready-to-use medium on 90mm plates supplemented with VTAP for the selective culture of Helicobacter pylori
- Code: 05011
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
BacterContact™ Tryptic Soy Agar + LTH
- Ready-to-use medium on 60mm plates for surface microbiological sampling with the addition of a neutralizer to neutralize surface disinfectants
- Code: 12008
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterContact™ Tryptic Soy Agar + LTHTh
- Ready-to-use medium on 60mm plates for surface microbiological sampling with the addition of a neutralizer to neutralize surface disinfectants
- Code: 12009
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterBagTM Dry Tryptone Soya Broth (TSB)
- High-quality dry medium, packaged in a transparent, lightweight, and convenient plastic bag. A highly nutritious general purpose medium for the cultivation of a wide variety of micro-organisms. For identification of Salmonella species from food samples in accordance with FDA BAM.
- Code: 10019
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterPlate™ Chocolate Agar with Isovilatex (CAIS)
- Non-selective culture medium for fastidious bacteria.
- Code: 05014
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
BacterPlate™ Mueller Hinton Agar (MHA)
- Ready-to-use medium on 90mm plates for testing the antibiotic sensitivity of easily cultured bacteria.
- Code: 05037
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
BacterContact™ Tryptic Soy Agar
- Ready-to-use medium on 60mm plates for surface microbiological sampling
- Code: 12007
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterPlate™ Chocolate Agar with Bacitracin (CAHI)
- Ready-to-use medium on 90mm plates for the selective culture of Haemophilus influenzae.
- Code: 05013
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.