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INTENDED USE
- TraceMedia™ Sheep Blood Agar is a medium used for cultivation and isolation of various microorganisms.
- It is also used to differentiate bacterial hemolysis types: alpha, beta, and gamma.
- The medium is employed for culturing and isolating infectious agents from respiratory specimens, throat swabs, nasal secretions, etc.
PRINCIPLE
- TraceMedia™ Sheep Blood Agar consists of Columbia Blood Agar Base supplemented with sheep blood, creating a medium that provides growth factors and allows for the determination of bacterial hemolytic reactions.
- On the surface of the agar plate, bacteria with a clear zone of hemolysis are classified as beta hemolysis.
- Bacteria that partially degrade hemoglobin, creating a greenish zone around the colonies, are classified as alpha hemolysis.
- Gamma hemolysis refers to no hemolysis observed on the surface.
READING THE RESULTS
- After incubation for the required time, observe the growth of colonies on the agar plate and their characteristics.
- Further identification of isolated bacteria must be conducted through appropriate tests.
- Samples related will be directly inoculated onto the agar plate.
- If the agar plate has been refrigerated, allow it to warm up to room temperature before inoculating the samples.
- Inoculate the samples onto the agar plate.
- Incubate the samples at 35 – 37°C, 5% CO2 for 16 – 24 hours.
BacterLab checks the quality of each product batch using standard strains from ATCC:
| Microorganism strain | Cultivation Reaction after 16-24 hours | Cultivation result at 35 – 37°C |
| Streptococcus pneumonia ATCC 49619 | Good growth, alpha-hemolytic bacteria | |
| Streptococcus pyogenes ATCC 19615 | Good growth, beta-hemolytic bacteria | |
| Enterococcus faecalis ATCC 29212 | Good growth, non-hemolytic bacteria |
Ingredients per 1 liter (Reference):
| Special peptone | 23 g |
| Starch | 1 g |
| Sodium chloride | 5 g |
| Agar | 10 g |
| Sheep blood | 5 – 7% |
| pH of the complete medium at 25°C: 7.3 ± 0.2 | |
*Please note that this composition is for reference purposes. Additionally, your company will prepare the components as required or according to the tender documents.
| Product name | Product code | Paking |
| TraceMedia™ Sheep Blood Agar | TM0722 | 10 Plates/Box |
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