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BacterChrom™ Mycoplasma Plates
- Ready-to-use chromogenic medium on 90mm plates for the detection of Mycoplasma bovis
- Code: 01034
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ Mycoplasma is a selective and chromogenic culture medium designed for the detection and differentiation of Mycoplasma bovis in samples such as nasal swabs and broncho-alveolar lavage. The medium aids in the diagnosis of bovine infections caused by M. bovis, which may present as pneumonia, arthritis, mastitis, or keratoconjunctivitis.
- This medium is primarily used in veterinary diagnostic laboratories and research environments.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ Mycoplasma utilizes chromogenic substrates that interact with specific enzymatic activities of M. bovis. Colonies of M. bovis are easily identifiable by their red coloration and characteristic “fried egg” morphology under a binocular loupe after 3–7 days of incubation at 37 °C in a CO₂ atmosphere. The medium also incorporates selective agents to inhibit the growth of non-target microorganisms, including Gram-positive and Gram-negative bacteria, yeasts, and molds. These features ensure high specificity, simplifying the identification process and supporting rapid and reliable diagnostic workflows.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Direct streaking sample onto the plate
- Incubate in aerobic conditions at 37°C for 3-7 days in a CO2
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| Mycoplasma bovis | red with a fried egg morphology |
| Gram (+) bacteria | mostly inhibited |
| Gram (-) bacteria | inhibited |
| Yeast and mould | inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Mycoplasma bovis ATCC 25523 | Incubate for 3 – 7 days at 35 – 37 °C, 5 – 10% CO2 | red with a fried egg morphology |
| Staphylococcus aureus ATCC 25923 | inhibited |
For 1 liter of medium
| Peptone and yeast extract | 25,0 g |
| Chromogenic mix and Selective mix | 0,3 g |
| Mineral salt | 6,3 g |
| Agar | 10,0 g |
| Growth factor | 2,2 g |
| Mineral salts | 1,0 g |
| Chromogenic mix and Selective mix (supplement) | 1,0 g |
| Growth factor (supplement) | 150 mL |
pH of the ready-to-use medium at 25°C: 7,6 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ Mycoplasma | 01034 | 10 plates/box or as per customer request. |
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