BacterPlate™ Mueller Hinton + Horse Blood + NAD (MHF)

Ready-to-use medium on 90mm plates for testing antibiotic susceptibility of difficult-to-cultivate microorganisms, requiring a high concentration of NAD+.
Code: 05029
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterPlate™ Mueller Hinton + Horse Blood + NAD (MHF) is a ready-to-use medium designed for antimicrobial susceptibility testing using the disk diffusion method.

PRINCIPLES

Meat extract and acid digest of casein supplies the required nitrogen, carbon, and vitamins in the medium. Starch absorbs any toxic metabolites produced. The medium contains low levels of thymidine and thymine and controlled levels of calcium and magnesium ions. Nicotinamide adenine dinucleotide (NAD) is added to make the medium suitable for use with more fastidious organisms such as Streptococcus pneumoniae and Haemophilus influenzae.

Antibiotic Susceptibility Testing (Kirby-Bauer Standard Method)

Use a pure culture from Tryptone Soy Broth (TSB) with turbidity matching the standard opacity of a barium sulfate suspension (0,5 on the McFarland scale).
Collect the inoculum using a sterile swab.
Inoculate the agar by streaking the swab 2 to 3 times across the entire agar surface to achieve uniform inoculum distribution.
Allow the plates to dry for 10 minutes before placing antibiotic disks.
Place the antibiotic disks with gentle pressure to ensure proper adhesion to the agar surface. Disks should be placed at least 15 mm from the plate edge and spaced sufficiently apart to avoid overlapping inhibition zones.
Incubate at 37°C for 18 to 24 hours.

Note: The Kirby-Bauer method is recognized as the most reliable and reproducible approach. However, other methods may also be employed, provided that the inoculum and inoculation procedures are thoroughly studied and standardized beforehand

RESULTS

Measure the inhibition zones.
Refer to the table for interpreting inhibition zones furnished by the suppliers of antibiotic disks in order to establish the correlation between the inhibition zone and the minimal inhibitory concentration (M.I.C.)

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation Conditions	Expected results
S. pneumonia ATCC 46919	18 – 24 hours, 35^oC, 5 % CO₂	Good growth, inhibition zone sizes according to CLSI